In situ electrochemical generation of nitric oxide for neuronal modulation

Abstract

Understanding the function of nitric oxide, a lipophilic messenger in physiological processes across nervous, cardiovascular and immune systems, is currently impeded by the dearth of tools to deliver this gaseous molecule in situ to specific cells. To address this need, we have developed iron sulfide nanoclusters that catalyse nitric oxide generation from benign sodium nitrite in the presence of modest electric fields. Locally generated nitric oxide activates the nitric oxide-sensitive cation channel, transient receptor potential vanilloid family member 1 (TRPV1), and the latency of TRPV1-mediated Ca2+ responses can be controlled by varying the applied voltage. Integrating these electrocatalytic nanoclusters with multimaterial fibres allows nitric oxide-mediated neuronal interrogation in vivo. The in situ generation of nitric oxide in the ventral tegmental area with the electrocatalytic fibres evoked neuronal excitation in the targeted brain region and its excitatory projections. This nitric oxide generation platform may advance mechanistic studies of the role of nitric oxide in the nervous system and other organs.

Publication
Nature Nanotechnology
Jimin Park
Jimin Park
Graduate Student

Materials Scientist | Neural Engineer | Makgeolli Maker

Atharva Sahasrabudhe
Atharva Sahasrabudhe
Graduate Student

Graduate student

Pohan Chiang
Assistant Professor at National Chiao Tung University
Florian Koehler
Florian Koehler
Graduate Student

Neuroengineer

Dekel Rosenfeld
Dekel Rosenfeld
Postdoctoral Associate

Postocotoral associate, focusing on organ modulation such as on demand control of hormone release from adrenal glands and investigation of the gut-to-brain axis.

Siyuan Rao
Siyuan Rao
Postdoctoral Fellow

Dr. Rao is a material engineer using her expertise in chemistry and biophysics to invent engineering tools for the study in neuroscience.

Polina Anikeeva
Polina Anikeeva
Associate Professor in Materials Science and Engineering
Associate Professor in Brain and Cognitive Sciences
Associate Director, Research Laboratory of Electronics

My goal is to combine the current knowledge of biology and nanoelectronics to develop materials and devices for minimally invasive treatments for neurological and neuromuscular diseases.

Related